Sentence examples for master gene expression from inspiring English sources
Table 2 shows some current therapeutic approaches that are aiming at EMT, most of them targeting kinase signaling pathways upstream EMT master gene expression.
Interesting new approaches include the previously mentioned vaccination against Brachyury-positive tumor cells and the transcriptional repression of EMT master gene expression by the anti-diabetic drug Metformin (Table 2) [3, 72, 143].
Inhibition of kinase signaling downstream FGFR3, ILK, Ras/MAPK or PI3K/AKT downregulates tumor formation, EMT master gene expression and invasive potential in colorectal, lung and pancreatic carcinoma cells in vitro, while in some models, re-expression of E-cadherin could be shown upon treatment with kinase inhibitors [137 140].
For all experiments unless otherwise stated, 20-μL reactions containing 5 μL sample were performed using universal master mix or gene expression master mix (Table S2) and the 7900HT fast real-time PCR system (all Life Technologies).
Investigating the cellular distribution of miRNAs deregulated in psoriasis we found that these master switches of gene expression are expressed in cells with key roles in the pathogenesis of psoriasis.
MicroRNA (miRNA) are master regulators of gene expression and impact cell proliferation, differentiation and apoptosis among others.
miRNAs have emerged as master regulators of gene expression in the nervous system where they contribute to neuronal network plasticity.
The p53 family of transcription factors includes p63, which is a master regulator of gene expression in epithelial cells.
MicroRNAs (miRNAs) are short single stranded RNA molecules, which serve as master regulators of gene expression.
Quantitative real-time PCR was carried out with TaqMan PCR Master Mix, TaqMan gene expression assays, and an Applied Biosystems 7900HT thermal cycler.
First strand cDNA samples from 18 different human tissues were purchased from OriGene, Inc. Universal human cDNA was purchased from BioChain Institute, Inc. Taq Man Universal Master Mix, TaqMan PreAmp Master Mix and gene expression assays were from Applied Biosystems.
