Sentence examples for master cell bank from inspiring English sources

The cells were obtained from the same master cell bank and the composition of the growing media was identical.

Master cell bank bacteria were diluted 1500-fold into Superbroth and recombinant protein was induced with 1 mM IPTG at an OD650 of 0.6.

Here we show that multiple cellular products currently being considered for therapy can be generated from a single master cell bank of this or any other clinically compliant iPSC line.

A single transformed colony was grown in Superbroth with ampicillin; bacteria were centrifuged at an OD650 of 1.3; master cell bank aliquots of bacteria in 30% glycerol/Superbroth were frozen and stored at −80 °C.

The process often lasts many months and can be divided into seven stages — host cell development, master cell bank establishment, protein production, purification, analysis, formulation, and storage and handling.

Due to the very low level of contamination, only an insert-specific PCR method was capable of tracing back the presence of the transposon in the source pDNA and master cell bank (MCB).

One seemingly well documented example is the requirement to test for replication competent retrovirus (RCR) which sponsors have to do on the master cell banks, retroviral supernatant lots as well as on the actual T cell product [ 45].

Master and working cell banks cultured were prepared using SC medium, and then further characterized to fulfill the cGMP guidelines for manufacturing biological products.

The best clone following GLP (good laboratory practices) is first fully characterised and then transferred to GMP (good manufacturing practices) to prepare the master and working cell banks.

From a vaccine production point of view, the characteristic of continuous growth is beneficial, since such cells have the potential for an infinite lifespan, and characterized and approved master and working cell banks can be established.

State-of-the-art transduction and culture technologies together with master and working cell banks should ensure the quality and the reproducibility of the medicinal cells (Scadden & Srivastava, 2012).