Sentence examples for master as per from inspiring English sources

Diluted cDNA samples were used for Real time PCR analysis with 200 nM of each primer mixed with SYBR Green PCR master as per manufacturer's instructions.

mRNA levels were assessed by Quantitative (q PCR on a Lightcycler 480 using Lightcycler 480 SYBR green 1 Master reagent (Roche) as per manufacturer's instructions.

They include such modern masters as David Hockney, Helen Frankenthaler, Jules Olitsky, Frank Stella and Anthony Caro.

Silicon masters serving as master stamps were fabricated using X-ray lithography as previously described.

Real time quantitation of EWS-FLI1 modulated targets NROB1, NKX2.2, GLI1, Cyclin D1, c-MYC, EZH2, TGFβIIR, KFL2 and EMT markers E-Cadherin, Vimentin, Slug, N-Cadherin and Fibronectin was done using SYBR green master mix (Fermentas GMBH, Germany) as per manufacturer's instructions.

Buddha like Christ and other masters moved from village to village as per the necessity with his disciples transmitting his message across India, Tibet and various parts of China.

The funding was by W.D.C.K. as per the requirement in the masters program.

Real-time PCR was carried out using KAPA master mix (KAPA SYBR® FAST Universal q PCR kit) as per manufacturer's instructions in triplicate on 7900HT Fast Real-Time PCR System.

Complementary DNA (cDNA) was created by adding 10 ng of RNA to the reverse transcription master mix and reverse-transcribed using the Superscript III Kit (Invitrogen) as per manufacturer's instructions.

The presence of miRNAs in 5 ng of cDNA was determined using miRNA-specific primers and SYBR Green master mix in the primary RB tumors and in cell lines as per manufacture's protocol.

The PDMS molds were made by casting from SU8 2150 (MicroChem, U.S.A) master fabricated on a silicon wafer by following the standard photolithography process as per manufacturer's instructions.