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Re-generate MAPGAPS profiles using the new master alignment of consensus sequences as a template and re-aligned group alignments.
The consensus sequence of each allele present within an isolate was assembled into a master alignment of all the Ldh alleles for a particular gene.
The input to the program is (1) a master alignment of all the sequences (only core domain was used for mcBPPS) (2) Seed profiles for each subgroup (3) a tree file giving the hierarchy.
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The resulting master alignment maintained all of the conserved motifs in register.
Based on a broad range master alignment, specific Trichoderma primers (ITSTrF/ITSTrR) were designed that comprise an approximate 650 bp fragment of the internal transcribed spacer region from all taxonomic clades of the genus Trichoderma.
In the next step, the initially obtained 1,634 candidate contigs were split into batches of 150 sequences and each batch was also added to the master alignment using MAFFT (-add option) resulting in 11 separate alignments.
At this step, an unweighted consensus master alignment was generated.
Noting that the previously resolved sequence logo of this template is only an approximation, we completed a more precise characterization using a master alignment.
We also produced a gap-free alignment by eliminating all positions harboring alignment gaps in the master alignment.
These kinds of microstructures can be fabricated by using the photolithography and wet-etching method step by step, but involves time-consuming design and fabrication process, as well as complicated alignment of different masters.
Thus, functional flexibility is much closer to BPS as conceptualized from a variety reduction perspective, where process variants and the alignment of variants against a master process play a central role.
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CEO of Professional Science Editing for Scientists @ prosciediting.com