Sentence examples for massively parallel technologies from inspiring English sources

This is particularly true for studies that require analysis of large numbers of samples, such as for temporal sampling or tissue/population surveys, which are not well suited for massively parallel technologies such as microarrays, SAGE, MPSS or next-generation sequencing (reviewed in [32]), due primarily to their high cost and technical complexity.

The need for high-throughput, low-cost sequencing drove the development of massively parallel technologies, also termed next-generation sequencing (NGS) technologies.

cDNA libraries made from the total RNA were sequenced using massively parallel technologies, and short reads (50 80 nucleotides) obtained were further processed for expression analysis and SNP calling.

These massively parallel technologies are rapidly replacing more traditional methods of DNA sequencing and typing; and although Sanger sequencing is still employed, it has largely become a complementary rather than standalone technology in many disciplines.

Current massively parallel technologies have high capital costs (ranging from $100,000 $1,000,000) requiring clinical laboratories to purchase and maintain large instruments to perform in-house genotyping.

Although Velvet [ 44] and ABySS [ 45] are popular assemblers for second-generation sequence data and can use Roche 454 pyrosequencing reads, they are primarily assemblers for genome sequence data from short-read platforms that rely on the high and even coverage depths afforded by these massively parallel technologies.

Affymetrix microarrays provide a massively parallel technology for identification of polymorphism in genes.

Our results have shown that low-depth genome sequencing using massively parallel technology provides sufficient sequence data for comprehensive repeat characterization even in a relatively large plant genome.

Today, variants called by massively parallel sequencing are commonly validated using either Sequenom genotyping, Sanger sequencing or resequencing using an alternate massively parallel technology (e.g. variants called from Illumina HiSeq data are validated with 454 sequencing); for examples see[ 1, 13- 15].

Compared with the traditional Sanger method, which is expensive and time consuming, next-generation sequencing (NGS) technologies or massively parallel sequencing technologies (e. g. Illumina/Solexa-based RNA-Seq technology) are much simpler and more cost-effective [ 17].

The development of massively parallel sequencing technologies, coupled with new massively parallel DNA enrichment technologies (genomic capture), has allowed the sequencing of targeted regions of the human genome in rapidly increasing numbers of samples.