Sentence examples for massively parallel sequencers from inspiring English sources
New generations of massively parallel sequencers have revolutionized the field of sequencing.
The current generation of massively parallel sequencers has led to a quantum leap in our ability to sequence genomes, so much so that 10-fold coverage of the human genome (30 Gb DNA sequence) can be obtained in a single run for no more than US$15,000 to US$20,000.
The dominant error mode for the most massively parallel sequencers is single base miscalls.
In the case of massively parallel sequencers, the high read error rate of the current technologies is a major problem.
The same massively parallel sequencers are simultaneously being used for whole-genome resequencing and comprehensive, genome-wide variant discovery in studies of somatic diseases such as cancer.
Initial selection ligand libraries, and libraries obtained after each selection cycle were sequenced using a massively parallel sequencer (HT-SELEX; Jolma et al., 2010, 2013).
To study genome-wide methylation patterns, we developed a method that leverages the selectivity of the MBD with the breadth and flexibility of massively parallel sequencing using the SOLiD sequencer (Fig. 1A).
The massively parallel sequencing using next generation sequencer (RNA-seq) provides a huge potential to revolutionize the field of pig transcriptome, owning to its abilities to discover extensive alternative splicing and identify large-scale novel transcripts at single-nucleotide resolution [ 12- 15].
These problems can be overcome by employing next-generation DNA sequencers based on massively parallel sequencing technologies, by which the cloning step is eliminated and sequence quantity is increased by orders-of-magnitude compared with that of conventional Sanger sequencers.
The next-generation sequencers are capable of massively parallel sequencing of thousands or millions of amplified DNA molecules in a single run and do not require the conventional cloning and amplification in bacteria when compared to the traditional Sanger capillary sequencers [ 6].
The complete coding sequence of the target genes was captured using a solution-phase Agilent SureSelect hybrid capture kit (AgilentTechnologies, Inc., Santa Clara, CA), and massively parallel sequencing was performed on an Illumina HiSeq 2500 sequencer (Illumina, Inc. San Diego, CA).
