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The results demonstrate how massively parallel reporter assays with libraries of designed sequences provide the statistical power required to address previously intractable questions about cis-regulatory function.
Here we introduce UTR-seq, a combination of massively parallel reporter assays and regression models, to survey the dynamics of tens of thousands of 3′ UTR sequences during early zebrafish embryogenesis.
Using chromatin interaction sequencing, massively parallel reporter assays (MPRA), and transgenic mice, we identified disease-linked, biallelic HAR mutations in active enhancers for CUX1, PTBP2, GPC4, CDKL5, and other genes implicated in neural function, ASD, or both.
Innovations in experimental and computational tools such as massively parallel reporter assays and deep learning have enabled the rapid screening of genomic variants for their causal impacts on splicing.
Recently developed massively parallel reporter gene assays combine next generation sequencing with high-throughput oligonucleotide synthesis to offer the capacity to test and mutationally perturb thousands of specifically chosen or designed cis-regulatory sequences in a single experiment.
Functional validation of these binding sites will require substantial functional dissection, which could be possible with massively parallel reporter assays [ 31].
Similar(44)
An LSPR-based multiarray nanochip for massively parallel detection was reported.
Anton is massively parallel, with 512 specialized processors working simultaneously.
Thinking Machines Corporation, in Cambridge, Massachusetts, sells massively parallel supercomputers.
In contrast, massively parallel computers chain together hundreds or thousands of standard microprocessors.
The code has been rewritten to run on modern hardware — so-called massively parallel computers.
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