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Wang, G.P., Ciuffi, A., Leipzig, J., Berry, C.C. & Bushman, F.D. HIV integration site selection: analysis by massively parallel pyrosequencing reveals association with epigenetic modifications.
In 2005 Stephan C. Schuster at Penn State University and colleagues published the first sequences of an environmental sample generated with high-throughput sequencing, in this case massively parallel pyrosequencing developed by 454 Life Sciences.
The Genome Sequencer FLX (GS FLX) is a sequencing system generating large amounts of sequence data through massively parallel pyrosequencing [2], [3], [4].
The product from the emulsion PCR was packed in ¼ of a PicoTiterPlate™ and the DNAs were loaded onto a 454/Roche GE-FLX for massively parallel pyrosequencing.
We used massively parallel pyrosequencing (i.e., "454 sequencing") to discover and characterize novel and known miRNAs expressed in primary cultures of normal human ovarian surface epithelium (HOSE) and in tissue from three of the most common histotypes of ovarian cancer.
We generated a reduced representation library (RRL) [25] to reduce the complexity of the white-tailed deer genome and a random shotgun library (RSL) to enable massively parallel pyrosequencing via the Roche 454 platform.
Massively parallel pyrosequencing transcended Sanger sequencing by enormously increasing throughput.
Specimens with discordant results were subjected to quantitative massively parallel pyrosequencing (MPP).
We have used massively parallel pyrosequencing to characterize the transcriptome of Zoarces viviparus.
Massively parallel pyrosequencing begins with fragmentation of the DNA and adaptor ligation.
However, in the library preparation steps the SOLiD platform resembles massively parallel pyrosequencing.
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