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AC: Audic-Claverie; CS: Correspondence Score; DABG: Detection Above Background; MPNS: Massively Parallel Nucleotide Sequencing; PSR: Probe Selection Region.
RNA-Seq exploits the rapid generation of gigabases of sequence data by Massively Parallel Nucleotide Sequencing, allowing for the mapping and digital quantification of whole transcriptomes.
Massively Parallel Nucleotide Sequencing (MPNS) allows the rapid generation of gigabases of sequence data at a relatively low cost per residue.
Although Rothberg et al. demonstrated a FET-based DNA sequencing system utilizing massively parallel nucleotide incorporation detection with millions of reaction wells using a single reference electrode, this assay is limited to reactions that occur in pulses through sequential addition of nucleotides.
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While there is mounting evidence that STRs are an important class of genetic variation with links to disease phenotypes and evolution of the human genome, their use in genetic studies has reduced with the advent of massively parallel single nucleotide polymorphism (SNP) analysis and genome-wide association studies (GWAS) [ 34, 35].
Haller, G. et al. Massively parallel single-nucleotide mutagenesis using reversibly terminated inosine.
In this study we compared two hybridization methods for target enrichment for massively parallel sequencing and single nucleotide polymorphism (SNP) discovery, namely Nimblegen sequence capture arrays and the SureSelect liquid-based hybrid capture system.
Recently, Spielmann et al. [ 48] used massively parallel sequencing to determine nucleotide sequences for each salivary RNA.
In an effort to achieve the first characterization of the genomes of SE of Canadian origin, we carried out massively parallel sequencing of the nucleotide sequence of 11 SE isolates obtained from poultry production environments (n = 9), a clam and a chicken, assembled finished genomes and investigated diversity of the SE genome.
Detection of endogenous cleavage-ligation products carrying internal deletion of hundreds to thousands nucleotides by massively parallel sequencing confirmed the catalytic properties.
They use a variety of analytic methods including shotgun massively parallel sequencing, targeted sequencing and single nucleotide polymorphisms together with complex algorithms [ 3, 4].
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