By utilizing a solid-state electrode, patterned with standard photolithography and evaporated onto the chip, we added the potential for massively parallel amplification detection.
However, current ion-sensitive field effect transistor methods for sensing nucleic acid amplification rely on establishing the fluid gate potential with a bulky, difficult to microfabricate reference electrode that limits the potential for massively parallel reaction detection.
There is a vast diversity of next-generation technologies, but these sequencing approaches generally use massively parallel amplification and detection strategies.
This method advances the potential for portable nucleic acid amplification detection; however, on-chip reference electrodes are still fairly large and difficult to integrate with a microchip, making it nearly impossible to achieve massively parallel, portable amplification detection.
Although Rothberg et al. demonstrated a FET-based DNA sequencing system utilizing massively parallel nucleotide incorporation detection with millions of reaction wells using a single reference electrode, this assay is limited to reactions that occur in pulses through sequential addition of nucleotides.
We have recently introduced massively parallel sequencing for mutation detection in clinical cancer specimens [20].
Massively parallel sequencing (MPS) detects single clones thereby being much more sensitive.
In this study, we evaluated the downstream effects of extended processing times on the reliability of aneuploidy detection by massively parallel sequencing (MPS).
This study demonstrates the potential of SMLM for characterising biosensing interfaces and as the transducer in a massively parallel, wide field, single molecule detection scheme for quantitative analysis.
