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The two main shortcomings of the state-of-the-art method of sorting chromosomes, specificity and the efficiency of fractionating a significant amount of chromosomes, are addressed by this work in the design of a massively parallel approach using magnetic beads binding to a chromosome-specific DNA probe.
The three genomes of Yoruban and Asian individuals, as well as a leukaemia genome were sequenced by a massively parallel approach using reversible terminator chemistry (Illumina-sequencing [6], [7], [8], [9]).
This drastic increase in both sample numbers and features per sample requires a massively parallel approach to data processing [ 6].
The introduction of NGS technologies was a critical development to allow for such a massively parallel approach in a time- and cost efficient way.
Our results for single vs. multiple chains indicate that 1PB, c2PB, c3PB, and REV methods that converge rapidly will benefit the most from a massively parallel approach to finding optimal networks.
These all use a massively parallel approach, producing hundreds of thousands to tens of millions of sequence reads at a time; however, they are much shorter than Sanger dideoxy reads.
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However, many recent reports have been published on massively parallel approaches to transcriptome sequencing [ 11- 13], largely using model organisms with available draft genomes to assist assembly [ 14, 15].
The recent emergence of massively parallel approaches for dense mutagenesis and the functional analysis of specific sequences [ 7] may enable a different paradigm, in which all possible variants of a clinically relevant gene are functionally tested in advance of ever having been observed in a patient.
Affymetrix microarrays in particular offer a massively-parallel approach to genotyping.
We report here on the application of a massively parallel sequencing approach utilizing sequencing-by-synthesis [ 10] as an efficient approach to generate ESTs.
In this paper, we proposed a massively parallel MCWT approach based on GPGPU to address this research challenge.
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