NGS-based HLA typing provides much higher resolution than Sanger sequencing and provides much higher sample throughput due to the massive number of sequencing reads generated in each run.
As a direct consequence of this aneuploidy, a massive number of sequence variants was identified, leading to at least 1,800 additional protein variants in S. pastorianus CBS1483.
Through a number of studies mentioned, undoubtedly, NGS represents a great powerful technology today which allows the massive number of sequencing reads to become available for only a short period and routinely be used for various genomewide association studies by aligning and mapping on the reference genome [ 9].
Some studies with massive numbers of sequencing reads produced from next generation sequencing technologies, include the Illumina HiSeq, Roche 454 and Life Technologies SOLiD, have been reported [ 26- 28].
First, the massive number of transcriptome sequences (approximately 4 million sequences in 16 tissues) could be used as a draft of the crab-eating macaque gene sequences.
Although NGS has been instrumental in the acquisition of a massive number of genomic sequences, the contribution of these sequences to understanding intra-host viral evolution have been modest, considering the enormous amount of genetic information generated by the technology [ 12].
From a massive number of nucleosomal DNA sequence data, we demonstrated that neighboring nucleosomes tend to be arranged with regular intervals.
Due to the massive number of 2.5 Mio read sequences obtained by 454 sequencing the de novo assembly of such datasets remains a computational and bioinformatic challenge.
Genome sequencing technologies allow the detection of a massive number of DNA polymorphism such as SNPs and Indels between closely related cultivars.
Although sequencing prices are rapidly decreasing, routine analyses of NGS data from a massive number of individuals for genomic evaluation still face serious challenges.
