Transcriptome libraries retain considerable potential for novel gene discovery despite massive recent cDNA and EST sequencing efforts; cDNA and EST evidence for these ES cell TUs had been limited or absent.
Moreover, RNA-Seq analysis facilitates research using species without a published genome sequence, such as litchi, by providing massive sequence data sets for molecular marker development, gene discovery, transcriptional analysis, and pathway enrichment analysis.
Massive sequencing of cDNA libraries will continue to produce raw materials for gene discovery, identification of polymorphisms (SNPs, EST-SSRs, INDELs, etc).
Massive sequencing techniques are among the new strategies used in functional genomics for gene discovery and molecular markers development in non-model organisms or in those species whose genomes have not been completely sequenced.
Moreover, massive parallelized sequencing technologies have made transcriptome studies, one of the most cost-effective methods for gene discovery, even more robust and efficient.
The chips are now widely used in gene discovery, cancer detection, drug discovery and toxicology.
Almost every week someone pops up with a gene discovery: the shopping gene, the gay gene or even the maths gene.
Both sides prefer the lower end of their range, since their methods of gene discovery tend to predict more genes than they believe exist.
Dr. Venter said he consulted his genetic profile every time a new announcement of a gene discovery came out.
Through massive gene transfer to the host nucleus, gene regulation is predominately conferred by nuclear genes.
