Like other neural cell cultures, insulin is required for hNSC growth, as withdrawal of insulin supplementation results in massive cell death and delayed cell growth.
Following prolonged growth arrest, massive cell death occurs eventually.
Conversely, we found that knockdown of WRAP53 impairs cancer cell growth by inducing massive apoptosis.
Consistent with previous studies [20], inactivation of Cdc13p induced growth arrest followed by massive cell death one day later (Fig. 1 and 2).
Overexpression of Beclin-1 in human cervical cancer cells has been reported to induce massive autophagic cell death and inhibit cancer cell growth [ 16].
In multiple breast cancer cell lines, DDX21 protein knockdown triggered cell cycle arrest as well as massive cell death and caused a significant reduction in anchorage-independent cell growth in vitro as well as in vivo.
The hard green stage, phase I, is characterized by rapid cell growth and elongation with a concomitant massive accumulation of organic acids, hydroxyl cinnamic acid, amino acids and tannins towards the end of this phase [ 15]-[ 19].
We have shown that, in the absence of exogenous growth factors, the 293-T " Phoenix" retrovirus packaging cell line undergoes massive cell death in a fashion strictly dependent on the availability of nutrients in the growth medium.
Despite these massive challenges there has recently been a renewed interest in measuring cell growth and several methods have emerged.
In the presence of TL/10 illumination, ApoG2 generated massive amounts of singlet oxygen and was more effective in inhibiting cell growth than in the absence of illumination.
