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Molecular masses were verified by mass spectrometry (Table 1).
Sample masses were verified via a MALDI-TOF mass-spectrometer (Applied biosystems, Carlsbad, California), HPLC purified (Waters, Milford Massachusetts), and lyophilized (Labconco, Kansas City Missouri).
Cleavage from the resin and removal of protecting groups was effected by a mixture of trifluoroacetic acid (95%), triisopropylsilane (2.5%) and water (2.5%).Peptides were purified by reverse-phase liquid chromatography using a linear acetonitrile:water gradient and masses were verified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry.
Their masses were verified by MALDI-TOF mass spectrometry.
Protein masses were verified using MALDI-TOF MS and SDS PAGE.
The fusion proteins with the expected molecular masses were verified by Western blotting using an anti-eNOS antibody.
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Geotechnical quality of one of these rock masses was verified by the back analysis of two slope failures.
All remaining explants were cultured for 16 weeks, during which formation of embryonal masses was verified under a stereomicroscope and recorded.
The body segment mass was verified by comparing segment mass percentages obtained from this model with previous data.
Finally, the effectiveness of support and excavation adjustments to maintain the stability of the rock mass is verified.
The linear relationship between the circulating composition and the charge composition and the inverse proportion relationship between the circulating composition and the charge mass are verified by the experiment.
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