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Exact(5)
Embryos were cultured to the blastocyst stage, 14 inner cell masses were isolated, and five ES cell lines originating from five separate embryos were derived, essentially as described for nonhuman primate ES cells (5, 6).
The Data Analysis DAA) software package (Bruker, Daltonics) was utilised to isolate masses and chromatograms of individual compounds, and masses were isolated from total ion chromatograms (TIC) by gating the retention time (RT) of individual compounds within an EIC.
Animals were euthanized with carbon dioxide, tumor masses were isolated, and tumor weight was measured.
Mononuclear cells from the liver, lungs and primary tumor masses were isolated by Percoll (Amersham, GE healthcare, Buckinghamshire, UK) gradient centrifugation.
The selected masses were isolated with a 1.5 m/z width in the linear ion trap and then fragmented using either linear ion trap with 35% relative collision energy or in the HCD (higher-energy collision-induced dissociation) collision cell, with a range of collision energies from 5%to75%5% relative collision energy.
Similar(55)
The QUICS method is also applicable to LC/MS applications where all ions eluting from the chromatographic system are fragmented rather than individual masses being isolated and then fragmented.
We suspected that the splenic mass was isolated metastasis from colon cancer at first.
Total RNA from micro-masses was isolated after 7, 14 or 21 days in culture using the Nucleospin RNA II kit (Macherey-Nagel, Düren, Germany).
Zonae pellucidae of expanded blastocysts were removed with brief protease (0.5%) treatment, and inner cell masses (ICMs) were isolated using immunosurgery (Mitalipov et al., 2006).
Taking advantage of the high resolution of the MS spectrometer (R > 30 40k), mass signals were isolated (Δ m/ z < 1 Da, monoisotopic peak) and mobility experiments were performed for each molecular ion as a function of the solvent condition and bath gas composition.
Several clones and the mass cell population were isolated and expanded for further analysis.
Related(20)
concentrations were isolated
numbers were isolated
earths were isolated
populations were isolated
constellations were isolated
population were isolated
masses were held
masses were observed
masses were computed
masses were considered
masses were offered
masses were balanced
masses were measured
masses were characterized
masses were embedded
masses were celebrated
masses were corrected
masses were calculated
masses were loaded
masses were sung
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