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The downslope movement of the ice masses cut and sculpted the U-shaped valley that attracts so many visitors to its scenic vistas today.
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To estimate the molecular mass of AMSs the concentrated supernatant (500 μL) was submitted to centrifugation at 4°C and 3 000 rpm for 1 h in an ultrafiltration membrane (Millipore Corp., USA) with a 10 000 Da molecular mass cut off.
The polymers were first dissolved in a cosolvent for 24 h (see Table 2) and then dialyzed against purified water for 3 days (membranes "Zellu Trans" with nominal molar mass cut off of 3,500).
Molecular-mass cut-off.
Another drawback to the IT is the low mass cut-off in product ion scans.
The authors found that a 5FU/kg lean body mass cut-off value of less than 20 mg/kg was a significant predictor of toxicity.
The highly sensitive product ion spectra of compounds 3a-e were obtained from a single-stage MS2 scan with abundant product ions and no low mass cut-off.
The key of this technique is the probe with a semipermeable membrane which has a molecular mass cut-off ranging from 5000 to 50,000 Da [53].
The dialysis tubes with a molecular-mass cut-off (MMCO) at 3.5 kDa were supplied by Thermo Scientific (Rockford, IL, USA), and those with MMCO 12.4 kDa by Sigma-Aldrich.
In the course of VLS, the compounds were minimally filtered by applying lower molecular mass cut-off of 220 Da, a polyphenols sub-structure filter, and a filter detecting chlorine atoms attached to aliphatic carbons.
The highly sensitive product ion spectra of compounds 3a-e obtained from a single-stage MS2 scan with abundant product ions and no low mass cut-off, are represented in Figures 1 and 2. Figure 1 a) ESI mass spectrum of compound 3a [M+H] + ion (m/z 328.80).
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