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Dynamic exclusion was set to 1 count and 30 s exclusion time with an exclusion mass window of ±20 ppm.
The mass window of the selecting quadrupole was 2 mass units.
SRM transitions were monitored with a mass window of 0.7 half-maximum peak width (unit resolution) in Q1 and Q3.
Dynamic exclusion was set to one count and 30 s exclusion time with an exclusion mass window of ± 20 ppm.
The dynamic exclusion list was restricted to a maximum of 500 entries with a maximum retention period of 90 seconds and a relative mass window of 10 ppm.
The dynamic exclusion list was restricted to a maximum of 500 entries with a maximum retention period of 180 s and a relative mass window of 15 ppm.
Similar(36)
The SWATH_MS approach does not select specific peptides for fragmentation, but instead scans repetitively incremental mass windows of about 25 mass units, every time fragmenting all the peptides that are present in each of these mass windows.
For collision induced dissociation tandem mass spectrometry, the mass window for precursor ion selection of the quadrupole mass analyzer was set to ±1 m/z.
Bins in smaller groups within are searched within the mass window (±0.4) of calculated mass and the RI window of the normalize bin of the larger group.
Due to the increased scan speed and mass window selectivity of the current mass analyzers, SRM can be simultaneously performed on multiple analytes.
Settings for cluster formation were first pass S/N 5 in 20% of all spectra and second pass S/N 2. Cluster mass window was 0.3% of the mass.
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