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Fat mass and lean mass were assessed using Dual Energy X-ray Absorptiometry DEXAA).
Its purity and molecular mass were assessed by electrospray mass spectrometry, high-performance liquid chromatography and amino acid analysis.
Anthropometric parameters (weight, length and head circumference) and fat mass were assessed at term and at 1, 3 and 5 months of corrected age.
C57BL/6J and db/db mice were treated daily with Boc5 for 4 weeks and a range of pharmacologic parameters, including hemoglobin A1c, intraperitoneal glucose tolerance, insulin tolerance, fasting insulin and leptin levels, food intake, body weight and fat mass, were assessed before and after the treatment.
Skeletal muscle mass (SkMM) and total fat mass were assessed using bioelectrical impedance.
Whole-body fat mass and lean body mass were assessed by dual energy X-ray absorptiometry.
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Total and regional body fat mass was assessed by dual energy X-ray absorptiometry.
Bone mass was assessed by dual-energy X-ray absorptiometry (DXA) and bone microarchitecture by micro-computed tomography (μCT).
Study design: Bone mass was assessed by densitometry and ultrasonography 60 days after surgery on 100-day-old female Wistar rats.
After the removal of acid, the digested samples were diluted to 5 ml, and Cd mass was assessed using inductively coupled plasma mass spectrometry (ICP-MS) (Agilent 7500, Santa Clara, CA, USA) according to the protocol as previously described [20].
Body mass was assessed by weighing individual animals on a laboratory scale.
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