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The dried chemical mass was dissolved in 10 ml n-hexane for instrumental analysis.
Sodium metasilicate of known mass was dissolved in distilled water using magnetic stirrer for about 1 h.
The mix of ~0.5-g mass was dissolved in 50 ml of high-purity ethanol and undergone to ultrasonic treatment (frequency, f = 4.2 kHz, time of the treatment, t = 20 min).
The resulting oily mass was dissolved in 1 ml of GC-grade n-hexane and 0.4 μl of the solution subjected to analysis on GC.
Dried A. aegerita fruiting bodies were crushed into a powder, and 50 g of the dried mass was dissolved and extracted three times with 1 litre of distilled water at 4°C.
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The zinc layer containing 152Tb, and other mass 152 impurities, was dissolved in 0.1 M HNO3/NH4NO3 at 80 °C.
To obtain free Fe3+ ions a precise mass of Fe3O4 nanoparticles was dissolved in a small volume of concentrated HCl under gentle heating [ 23].
The crucibles were subsequently re-weighed to obtain the mass of ash, which was dissolved in 10 ml of trace element analysis concentrated nitric acid and sonicated for 30 min to ensure stabilisation.
For PET imaging studies using mass amounts, afatinib (1.0 mg) was dissolved in DMSO (1.0 mL) and diluted with formulation solution (7.09 mM NaH2PO4 in 0.9% NaCl, w/v in water, pH 5.2) to the appropriate concentrations (40, 120, 400, and 1,200 nM), and after addition of 50 μL [18F]afatinib to 50 μL of these solutions, they were injected as a IV bolus.
Transport buffer contained 20 mM HEPES/KOH, pH 7.3, 110 mM potassium acetate, 5 mM sodium acetate, 2 mM magnesium acetate, 1 mM EGTA, and 2 mM DTT. Amino-derivatized dextran (molecular mass 500 kDa; Invitrogen, Germany) was dissolved in 0.1 M NaHCO3, pH 8, and fluorescence labeled with a 5-fold excess of ATTO647 succidinimidyl ester (ATTO-TEC GmbH, Siegen, Germany).
Thus, the biofilm mass of all tested strains was dissolved with SDS and measured.
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