S.P. designed all experiments and performed evolution, RT-qPCR, enzyme assays, gene deletions, genome sequencing library generation, mass spec verification and measured growth phenotypes.
An aliquot from each reaction mixture at 0 h was prepared to allow matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometric verification of the synthetic peptides prior to incubation.
a HPLC detection revealed a peak at RT 18.377 min (λ = 405 nm) with UV Vis spectra in the acid mobile phase, and mass spectra verification revealed a molecular ion peak (M−) at m/z 231.0299 (C12H8O5) consistent with HOBB.
A sejm began with a solemn mass, a verification of deputies mandates, and election of the Marshal of the Sejm (also known as the Speaker).
Pearson, G. S. The Search for Iraq's Weapons of Mass Destruction: Inspection, Verification and Non-Proliferation (Palgrave/Macmillan, 2005).
We also used an alternative means to verify our Cdc45 measurements in which we immunoprecipitated Cdc45, estimated the amount immunoprecipitated using immunoblots, and subjected the immunoprecipitated Cdc45 to quantitative mass spectrometric (QMS) verification.
SEDT incorporates system budgets, mass, and power as verification parameters, along with characteristic trend equations (CTEs).
In this study, ethanol vapour was ionized by a UV discharge lamp and the RIT was used as a mass analyzer during the verification experiment.
In this manual verification the mass error, the presence of fragment ion series and the expected prevalence of C-terminus containing ions (Y-type) in the high mass range were all taken into account.
DK participated in sample preparation, mass spectrometry, data analysis, verification of methodology, and drafted the manuscript.
