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These findings tend to suggest that the enzyme had high affinity toward high-molecular mass substrates containing α-1,4 and α-1,6 linkages.
The low molecular mass substrates 2′- 4-methylumbelliferyl)- N-acetylneuraminic acid (MU-NANA), 3′-sialyl- N-acetyllactosamine (3SLN) and 6′-sialyl- N-acetyllactosamine (6SLN) contained one sialic acid residue per molecule.
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However, ethanol fermentation processes exhibit complex behavior and nonlinear dynamics with respect to the cell mass, substrate, feed-rate, etc.
The model links mass substrate fluxes to all of these metabolites via a stoichiometrically precise reaction network that includes and therefore balances NAD+ and NADH.
For tuber α-amylase, high-molecular-mass substrates containing the α-1,4-linkage were better substrates for the enzyme.
The chimeric chitinases had similar activities towards soluble substrate but higher hydrolytic activity than the native chitinase on high molecular mass insoluble substrates such as ground chitin or chitin-rich fungal cell walls.
Metabolomics is the quantitative analysis of a large number of low molecular mass metabolite, substrates or products in metabolic pathways.
Organic vapor adsorption isotherms are measured on in situ grown carbon nanotube (CNT) films using piezoelectric GaPO4 crystal microbalances as mass sensing substrates.
This result is reasonable because some GH10 xylanases have activity on low molecular mass cellulose substrates, particularly aryl cellobiosides [ 32].
For substrate mass balance, the substrate concentration (S) is written as (4) S = S o − SE − P. Substituting (4) into (2) gives (5) d SE d t = k 1 (S 0 − SE − P ) × E − k − 1 SE − k 2 SE.
To ensure resolution of the Phos-Tag gel, the volume of the sample loaded from the reactions containing high concentration of substrate (lanes 1 12) was adjusted to ensure equality in the total mass of substrate plus product (total eIF2a) in each lane.
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