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The false discovery rate was estimated to be <1% at the mass spectrum level and <2% at the peptide level.
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The confirmation by a full-scan electron impact (EI) mass spectrum is possible if residual level in a sample is >5 μg/mL.
In particular, the selection of the peak threshold in mass spectrum analysis is difficult and somewhat arbitrary, and the level of biomarker candidates is not fully quantitative.
However, contribution of cAMP below the levels required for obtaining a tandem mass spectrum cannot be ruled out.
These high-dimensional profile data is very similar to SNP (sequence data), gene expression (transcriptome), mass spectrum (proteome), and small molecules (metabolome) data in different levels.
The MTS response returns to baseline levels within 3 min. The mass spectrum obtained from the MTS desorption peak is shown in Figure 3c.
The level of significance of the association between a microarray marker and a specific mass spectrum marker was determined by introducing background mass spectra distributions estimated by all human protein sequences in the SWISS-PROT database.
The confidence in the peptide mass fingerprinting matches was based on the score level and confirmed by the accurate overlapping of the matched peptides with the major peaks of the mass spectrum.
d Mass spectrum of Methylnaphthalene.
tandem mass spectrum.
full scan mass spectrum.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com