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d Mass spectrum collected via APT voltage-pulsed mode of MeCpPtMe3 deposited onto a Si micropost array substrate cryogenically cooled to 113 K in the FIB/SEM and environmentally transferred under high vacuum into the atom probe.
The APT time-of-flight mass spectrum collected under voltage pulsing mode of MeCpPtMe3 that has been cryogenically deposited as a thin film within the FIB/SEM and cryogenically transferred to the LEAP using the ETH, shows only a single mass peak group centered at 319 Da (Fig. 2d), the mass of which is consistent with the intact MeCpPtMe 3 + molecule (C9H16Pt = 319 Da).
Identification of compounds was carried out by comparing the mass spectrum collected from the Wiley library and retention times and mass spectra to those obtained from the analyses of authentic standards (PAHs were purchased from Neochema, Bodenheim, Germany; dioctyldiphenylamine was purchased from Chemos GmbH, Regenstauf, Germany).
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Figure 2 Inter-instrument comparability of dixyrazine-specific tandem mass spectra collected on different instrumental platforms.
Mass spectra collected by FIB-SIMS reveal the counter mass transportation of inward oxygen diffusion and outward diffusion of aluminium.
The APT ToF mass spectra collected under voltage-pulsing mode are also qualitatively similar for both the IBAD (Fig. 2b) and the EBAD (Fig. 2c) specimens, although the EBAD specimens ran slightly longer before fracture.
Mass spectra collected from each point within the raster are used to assemble the molecular images in correlation with their x- and y- positions.
Mass spectra collected by MALDI-QqTOF and MALDI-ion trap mass spectrometers were analyzed by the computer search engines ProFound (http://prowl.rockefeller.edu/prowl-cgi/profound.exe), Xproteo (http://www.xproteo.com) and GPM (http://prowl.rockefeller.edu/tandem/thegpm_tandem.html) using the NCBI non-redundant mouse protein database.
Spectra values represent the total number of mass spectra collected by MS and matched to the protein using proteomics software.
The total number of mass spectra collected and matched to a specific protein is indicated by the 'spectra' value.
Mass spectra collected for the intact protein complexes were recorded on a QSTAR XL mass spectrometer (MDS Sciex, Concord, Canada) modified for high-mass detection (Sobott et al, 2002).
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