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SDS-PAGE and mass spectrum analysis showed that BMB1073 could express HaCad1 and Cry1Ac proteins together.
The above results are basically in accordance with mass spectrum analysis and far more specific.
The sensing mechanism was based on nucleophilic addition between CN− and indolium group, which was confirmed by 1H NMR and mass spectrum analysis.
To explore potential cofactors that might stabilize TRIM24, we performed tandem affinity purification combined with mass spectrum analysis of TRIM24-containing complexes in LNCaP cells and identified a number of proteins, ranked among top of which were TRIM family proteins TRIM28 and TRIM33 (Supplementary Table 1).
Xin Zeng performed the HPLC and mass spectrum analysis.
f Mass spectrum analysis of purified recombinant LL-TIF on a MALDI-TOF mass spectrometer.
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Combined with SDS-PAGE electrophoresis and a subsequent mass spectra analysis, β-catenin, a transcription factor that promotes CD36 transcription, was found in the complex.
Membrane-depleted nuclei purified from NB4 cells induced to differentiate with ATRA in the presence of forcedly down-modulated Vav1 were subjected to 2D-DIGE followed by mass spectra analysis.
Mass spectra analysis was performed using the CAMECA Integrated Visualization and Analysis Software (IVAS®), version 3.6.6.
The lipopeptides mixture was dissolved in Milli-Q water and filtered through a 0.2 μm membrane filter (PALL Gelman Laboratory, New York, USA) to obtain a 500 μg/ml stock solution for mass spectra analysis.
Mass spectra analysis was performed using a Tof-spec SE (Micromass, Manchester, UK) or MALDI-Micro MX mass spectrometer with PSD technology (Micromass, Manchester, UK).
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