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Triple-quadrupole mass spectroscopy methods were used to measure plasma ECS analogs.
Despite the relatively small subject cohort, tight plasma ranges and statistical significance (Figure 1) was achieved by using highly sensitive triple quadrupole mass spectroscopy methods.
To determine whether a similar set of multi-protein complexes exists in Drosophila we utilized affinity purification and mass spectroscopy, methods that have proved successful for analysis of kinetochores in other organisms [3], [6].
Similarly, other global 5-hmC level quantifications can be accomplished by high-performance liquid chromatography (HPLC)–mass spectroscopy methods.
Despite these considerations, mass spectroscopy methods benefit from assessment of soluble markers, which are likely to be more abundant than cancer cells.
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In the first case, a mass spectroscopy method developed by the Hildebrand laboratory successfully identified four HLA-A*0201 HLA-A*0201 WNV CD8+ T cell epitopes [9].
Our internal standard and mass spectroscopy method are specific for detection of a single LG-lysyl adduct with an m/ z equivalent to that of the lactam structure, but the initial Schiff base intermediate of LG-lysyl adducts can oxidize to form other structure such as hydroxylactam or intraprotein or protein DNA cross-links, which would go undetected in our method.
Using filtrates from fermented kimchi produced using Leuconostoc mesenteroides LBP-K06 starter cultures, we previously demonstrated a combined chromatographic fractionation and gas chromatography-mass spectroscopy method for screening bioactive CDPs.
On the basis of experimental data obtained by thermogravimetric analysis, X-ray analysis and laser mass-spectroscopy methods, conclusions about the formed oxide layer compositions were made.
Recent improvements in software, hardware, and instrumentation design have made liquid chromatography and tandem mass spectroscopy (LC MS/MS) methods optimal choices for the determination of many oxidative stress markers.
Protein bands from three separate purifications were submitted for in-gel digestion and peptide mass spectroscopy (see Methods & Additional file 1 for details).
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