On the basis of the analysis of sterol composition by chromatographic and mass spectroscopic methods, sterol biosynthetic pathways in T. cruzi amastigotes and epimastigotes were partially constructed.
Here, gel electrophoretic, mass spectroscopic, and chemical kinetics methods were used to provide evidence that medicinally relevant concentrations of 4 rapidly form covalent adducts with abasic sites in double- and single-stranded DNA under physiological conditions.
In the work described here, we employed gel electrophoretic, mass spectroscopic, and chemical kinetics methods to provide evidence that medicinally relevant concentrations of 4 rapidly form covalent adducts with abasic sites in double- and single-stranded DNA under physiological conditions.
The structure of this isolated compound was determined by a combination of spectroscopic analyses, including IR, H and C NMR, hydrogen-hydrogen correlation spectroscopy (H-H COSY), heteronuclear multiple quantum coherence (HMQC), heteronuclear multiple bond coherence (HMBC), mass spectroscopic data, and chemical methods.
Potent fractions were chosen for further identifications using the chromatographic and spectroscopic methods as follows: a- Mass spectroscopic (MS) analyses of potent fractions The biologically potent fractions of E. crassipes were analyzed by Mass spectra (MS).
Plasma quinine/3-hydroxyquinine and nevirapine were determined using a validated high-performance liquid chromatography method with fluorescence detection, 20 and liquid chromatographic-tandem mass spectroscopic, 21 respectively.
Both methods are isotope-specific, and the combination of NMR and FTICR mass spectral data provides more useful spectral information on complex unknowns at the molecular level than any other spectroscopic method at present.
In other projects computers were used to analyze the massive data sets generated by mass spectroscopic or even tandem (multiple and simultaneous) mass spectroscopic analyses of proteins or small metabolites in biological samples.
Figure 4 shows the result of the mass spectroscopic analysis.
Plasma density and temperature were measured using spectroscopic method.
