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It was also confirmed by mass spectrophotometry using LC-MS/MS apparatus.
The molecular mass of the native enzyme was determined by matrix assisted laser desorption ionisation time-of-flight (MALDI-TOF) mass spectrophotometry, using Voyager DE-STR (Applied Biosystems, USA) equipped with a 337 nm nitrogen laser.
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The most common one used is spectrophotometry using selective reagents.
The silicic acid content was measured by spectrophotometry using molybdenum blue assay[13].
Permeabilization was measured by fluorescence microscopy and spectrophotometry using SG uptake assay.
The concentration of RNA was determined by spectrophotometry, using Nanodrop-1000 (Nanodrop Technologies, Wilmington, DE).
Nucleic acid concentrations were determined by spectrophotometry using the Nanodrop instrument (Thermo Scientific, Wilmington, DE).
Concentration and purity of the RNA extracted was determined by spectrophotometry using a 1∶10 diluted sample.
Total DNA concentration was determined by spectrophotometry using Nanodrop.
Reaction product was detected by spectrophotometry using microtiter plate reader.
Protein concentrations were determined by spectrophotometry using Bradford reagent.
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