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We investigated a variant of desorption/ionization on porous silicon (DIOS) mass spectrometry utilizing an aqueous suspension of either porous silica gel or porous alumina (pore size of 60 and 90 Å, respectively).
Here, we conducted quantitative mass spectrometry utilizing stable isotope labeling (SILAC) analysis to profile candidate SRC-substrates induced by the CSF-1R tyrosine kinase by comparing the phosphotyrosine-containing peptides from cells expressing either CSF-1R or a mutant form of this RTK that is unable to bind to SFKs.
Synovial fluid concentrations of florfenicol, gamithromycin and tulathromycin were measured with high-pressure liquid chromatography tandem mass spectrometry utilizing a LTQ ion trap mass spectrometer (Thermo Scientific, San Jose, CA, USA) coupled to an Agilent 1100 series pump and Autosampler (Agilent Technologies, Santa Clara, CA, USA).
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The UV-MALDI-Orbitrap mass spectrometry was utilized in solid-state and semi-liquid colloidal state, involving the laser ablation at λex = 337.2 nm.
The polymers exhibited a predominantly trans microstructure and matrix-assisted laser desorption/ionization mass spectrometry was utilized to determine polymer end groups and to calculate the absolute molecular weights.
To further characterize the UV-induced chemical modifications to ALDH3A1, peptide mapping with MALDI-TOF mass spectrometry was utilized.
We have not detected β2-microglobulin reported previously in CA deposits by immunohistochemical analysis [17], even through we were able to detect small traces of bacterial proteins due to the high sensitivity of the liquid chromatography-electrospray ionization mass spectrometry technique, utilized in this study.
Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry was utilized to confirm successful synthesis of PAs.
To further understand the differences in binding selectivity, MALDI-TOF mass spectrometry was utilized to analyze the precise molecular weights of the arsenic zinc finger complex.
Because of the small amounts of substrate obtained from the previous step, mass spectrometry was utilized to monitor generation of the CMP-activated sugar product.
However, the increased sensitivity of ABPP and mass spectrometry techniques utilized in our study permitted the identification of both plasmin and urokinase-type plasminogen activator in healthy individuals.
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