Sentence examples for mass spectrometry sample from inspiring English sources

Mass spectrometry sample preparation was described in our previous report (Kniazeva et al., 2012).

Procedures for quantitative mass spectrometry sample preparation and data analysis were as previous described [ 42, 43].

Detailed description of reagents, peptide synthesis, molecular cloning, protein expression and purification, and mass spectrometry sample preparation.

Due to unspecific binding of histones to the eppendorf tubes, we used eppendorf tubes with low binding affinity during mass spectrometry sample preparation.

Detailed description of reagents, peptide synthesis, molecular cloning, protein expression and purification, and mass spectrometry sample preparation is provided in the Supporting Information.

For mass spectrometry, experiments were performed as described above with the following modifications: 1,250 μg of extract was used per replisome assembly reaction; DDK phosphorylation step and extract incubation step were both extended to 30 min; and 4 replisome assembly reactions were pooled for each mass spectrometry sample.

Variability in the number of quantified proteins reflects variability in protein measurement and mass spectrometry sampling.

We did not detect the other major component of the linear polyubiquitination complex, a protein known as heme-oxidized IRP2 ubiquitin ligase-1 (HOIL-1), in our mass spectrometry samples.

Myosin is known to connect with adhesion plaques, and one isolated report has indicated that myosin heavy chains under some conditions can be tyrosine phosphorylated [25], but, we also cannot rule out the possibility of myosin contamination of the mass spectrometry samples given its abundance in smooth muscle.

For mass spectrometry samples were treated and analyzed as described [ 48].

For mass spectrometry, samples were prepared with unlabeled mRNA and incubated with and without RelEwt.