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Citrulline concentrations were determined by stable isotope dilution tandem mass spectrometry (coefficient of variation, <10%).
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Plasma F2-isoprostane and 24-h urinary 20-hydroxyeicosatetraenoic acid levels (markers of systemic oxidative stress) were measured by gas chromatography-mass spectrometry (interassay coefficients of variation 5.6 and 10%, respectively) (6– 8).
Spun serum from these samples was shipped to the Southwest Research Institute (San Antonio, TX) as a quality assurance check and analyzed using liquid chromatography tandem mass spectrometry; the Spearman rank correlation coefficient for paired PFOA concentrations measured by the two laboratories was 0.89.
Serum creatinine was analyzed by a colorimetric assay (compensated Jaffe reaction), calibrated by Isotope Dilution Mass Spectrometry (IDMS), with an inter-assay coefficient of variation of 1.75% at a mean level of 104 µmol/l mg/dl (Roche).
CV: coefficient of variation; DHEAS: dehydroepiandrosterone sulphate; MS: mass spectrometry; PHV: peak height velocity; RIA: radioimmunoassay; TW II: Tanner Whitehouse.
There was a weak but significant correlation between mycolactone measured by mass spectrometry at baseline with time to complete healing (Pearson's correlation coefficient 0.24; p = 0.05).
Secondary ion mass spectrometry was used to measure the lattice and grain boundary diffusion coefficients for the two species.
The grain boundary oxygen diffusion coefficients were measured by the isotopic tracer profiling technique using secondary ion mass spectrometry.
Diffusion coefficients of hydrogen in Si-QDSLs for several treatment temperatures were estimated by secondary ion mass spectrometry (SIMS).
The main method used has been mass spectrometry.
Tandem mass spectrometry.
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