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The eluent is concurrently introduced into the mass spectrometer via electrospray ionization (ESI).
Analyte eluted from the capillary column was introduced into the mass spectrometer via a jet separator.
The eluent is concurrently introduced into the mass spectrometer via electrospray ionization (ESI) or nanospray, which is a derivative of ESI.
For GC-IRMS analysis, in which carbon isotopic ratio is measured for specific saturated hydrocarbon, a trace GC was coupled to a Delta V isotope ratio mass spectrometer via a combustion interface, and a ComFlow IV unit, all ThermoFisher.
The stable hydrogen isotopic composition of CH4 was analyzed by isotope ratio monitoring gas chromatography/mass spectrometery (irm-GC/MS) using a Thermo Finnigan Trace Ultra GC, connected to a Thermo Finnigan DELTA V Plus mass spectrometer via Thermo Finnigan GC-Isolink interface as reported previously [13].
The analysis involved online transfer of samples from a high temperature conversion reactor (containing an empty ceramic tube covered with graphite layer that was kept at a temperature of 1440°C) in which compounds were pyrolyzed to molecular hydrogen, carbon, and carbon monoxide, prior to their transfer into the mass spectrometer via Conflow IV interface.
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Peptide mixtures were subjected to LC-MS/MS analysis on a Finnigan LCQ (PTRL West) or 4000QTRAP (Applied Biosystems) ion trap mass spectrometers via reversed phase HPLC nano-electrospray ionization.
Digested samples (2 μl) were introduced into a Waters Q-Tof micro-mass spectrometer via a Waters CapLC system that was configured with a PepMap C18 (LC Packing, 300 μm ID × 5 mm) preconcentration column in series with an Atlantis (Waters) dC18 NanoEase (75 m × 150 mm) nanoscale analytic column.
Peptides LC-MS/MS analyses were carried out using a custom-built automated capillary LC system coupled online to an LTQ mass spectrometer (Thermo Scientific) via a nanoelectrospray ionization interface manufactured in-house.
The HPLC (High-performance liquid chromatography) system was coupled to a QExactive mass spectrometer (Thermo Fisher Scientific Inc) via a EasySpray source.
For the long gradient experiments, the LC was connected to a Q Exactive mass spectrometer (Thermo Scientific) via a nanoelectrospray ion source (Proxeon).
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