Detection was performed on tandem mass spectrometer using electrospray ionization source in positive mode by multiple reaction monitoring.
Mass spectra were obtained with a Waters nanoACQUITY UPLC coupled to a Thermo Exactive Orbitrap mass spectrometer using electrospray ionization (ESI-MS) operated in negative ion mode.
Samples were analyzed on a Thermo-Finnigan Trace DSQ™ fast-scanning single-quadrupole mass spectrometer using electron impact ionization (EI) and operated at unit mass resolving power.
Fig. 1 Schematic picture of a Nier-type isotope ratio mass spectrometer using a gas source analytical medium (in this example carbon dioxide, CO2).
Sample was introduced into the mass spectrometer using a Harvard syringe pump (Holliston, MA) at a flow rate of 5 μL min−1.
The HPLC-separated antioxidant compounds were identified using HPLC-photodiode array coupled to mass spectrometer, using a reference mass for determining accurate masses.
The lipid profiling was carried out on Waters Q-Tof Premier mass spectrometer using ESI+ mode.
Briefly, hair was wrapped in silver foil and admitted into the combustion chamber of a mass spectrometer using a Carlo Erba AS 200-LS autosampler.
Peptides were eluted from the C-18 column into the mass spectrometer using a linear gradient of 5 60% Buffer B over 60 min at 400 ul/min.
In brief, samples were collected with an LTQ – Orbitrap hybrid mass spectrometer, using a top-ten method, a dynamic exclusion repeat count of 1, and a repeat duration of 30 sec.
All MS experiments were conducted on a 4000 QTRAP hybrid triple-quadrupole linear ion trap mass spectrometer using Analyst version 1.4.1 software (Applied Biosystems, http://www.appliedbiosystems.com/) equipped with a TurboIon source used in positive ion electrospray mode.
