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All analyses were conducted on an Agilent HP 1260 Infinity Series liquid chromatograph equipped with a DAD system (Agilent Technologies, Santa Clara, CA, USA) coupled to a Q-TOF mass spectrometer model G6540B (Agilent Technologies) with a Dual ESI source.
Peptides from each sample were then separated by reversed phase nano-HPLC-Chip technology (Agilent Technologies) online-coupled with a 3D ion trap mass spectrometer (model Esquire 6000, Bruker Daltonics).
For GC MS, model GC-6860N direcoupledupled with mass spectrometer, model MS-597MSD was used.
(6) Helium and neon concentrations and the helium isotope ratios were measured with a noble gas mass spectrometer, model MM5400 (Micromass UK Ltd., Manchester, UK).
Agilent 1100 LC has been coupled with Bruker make mass spectrometer model Esquire 3000.
All isotope enrichments were measured on a gas chromatograph mass spectrometer (model 6890/5973; Hewlett-Packard, Palo Alto, CA).
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To standardize the fingerprint of E. hirta extract, GC/MS chromatographic analysis was performed on a thermogas chromatograph-mass spectrometer (model Shimadzu 2010) equipped with DB-5 capillary column (30 m long. 0.25 mm i.d., film thickness 0.25 μm).
For verification of RIA measurements of 5αP, 3αHP, and progesterone, portions of TLC-separated extracts from four tumor tissues were tested with both RIA and GC/MS (Hewlett-Packard GC-Mass Spectrometer, model 5790A/5970A, used in the selected ion mode (SIM) with a DB-1MS 12-m × 0.2 mm × 0.33 μm cross-linked methyl silicone capillary column).
We identified volatile components with gas chromatography (Agilent model 7890A) coupled with a mass spectrometer (Agilent model 5975C) in electron ionization mode comparing retention times and spectra with that of pure compounds.
The analysis was performed on a gas chromatograph coupled to a mass spectrometer (Agilent Technology, model 6890N); this was coupled to a mass selective detector (model 5973) with a DB-5HT capillary column (15 m in length, 0.25 mm internal diameter, and 0.10 μm film thickness).
Gas composition was analyzed by combining a Varian CP-3800 gas chromatograph with Ar as the carrier gas and a Quadrupole Mass Spectrometer (QMS) model Pfeiffer Omnistar 422.
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