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The ability to operate at higher pressure enabled a new tandem ion funnel mass spectrometer interface design that can accommodate a greater gas load (e.g., from an ESI source).
We have constructed a mass spectrometer interface for planar geometries that takes advantage of atmospheric pressure gas dynamic principles, rather than ion focusing, to minimize ion losses from diffusion in the analyzer and to maximize total ion transport into the mass spectrometer.
The entire chromatographic effluent (10 μl) was passed through the mass spectrometer interface for subsequent detection.
The mass spectrometer interface is compatible with any microfluidics channel or device that can be terminated by a capillary outlet.
Ion formation is initiated by applying a potential of ∼−700 V to the heated capillary of the mass spectrometer interface.
Similar(55)
All methods utilized a Waters ACQUITY ultra-performance liquid chromatography (UPLC) and a Thermo Scientific Q-Exactive high resolution/accurate mass spectrometer interfaced with a heated electrospray ionization (HESI-II) source and Orbitrap mass analyzer operated at 35,000 mass resolution.
In this work we describe the design and operation of (1) a linear quadrupole mass filter and (2) a quadrupole ion trap mass spectrometer interfaced, in each case, with a membrane introduction/fluid control system and packaged for underwater operation.
The UPLC-MS/MS was performed for a non-targeted metabolomics analysis based on a Waters ACQUITY ultra-performance liquid chromatography (UPLC) and a Thermo Scientific Q-Exactive high resolution/accurate mass spectrometer interfaced with a heated electrospray ionization (HESI-II) source and Orbitrap mass analyzer operated at 35,000 mass resolution.
Mass data were analysed with a prOTOFTM 2000 mass spectrometer interfaced with TOFWorksTM software (PerkinElmer/SCIEX).
Specifically, we have acquired a research grade hybrid quadrapole ESI-MS/MS mass spectrometer interfaced with a capillary liquid chromatograph (cap-LC).
The GC-MS analyses was carried out using Hewlett-Packard HP 5973 mass spectrometer interfaced with an HP-6890 gas chromatograph with an HP5 column (30 m × 0.25 mm id, 0.25 μm film thickness) and an MS detector.
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