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Here we describe the evolution of tandem instruments based on the CFR, from its introduction in 1993 to the current commercial TOF2 mass spectrometer from Shimadzu Corporation, and briefly review the history of TOF/TOF instruments.
Importantly, the facility is poised to enter into a new phase of expansion with the recent delivery of a 2 MV 14C tandem accelerator system from High Voltage Engineering (HVE) and a stable isotope ratio mass spectrometer from Micromass Inc. for combustion of organic samples and isotopic analysis.
The system includes CO2 and N2 cylinders, two MFC (calibrated for CO2 and N2 flow rates), two check valves, and bypass tube (for calibrating the gases concentration detected by mass spectrometer from the inlet gas compositions).
The a.s. and selected additives (as far as analytical methods were available or could be established within the scope of the project) were analysed by direct injection of the samples into a liquid chromatographic system with tandem mass spectrometer (HPLC 1260 Infinity from Agilent Technologies coupled via an electrospray interface to an API 5500 tandem mass spectrometer from AB Sciex).
The system included two gas regulators with dual pressure gauges and output control valves, two mass flow controllers (one was calibrated for CO2 flow and the other was calibrated for N2), two check valves (to control the flow in one direction), and a bypass line (for calibrating the mass spectrometer from the feed gas mixture).
A Quattro Premier XE tandem mass spectrometer from Waters-Micromass was utilized for analyte detection.
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A brief account is given of the history of dynamic mass spectrometers from the time of their advent.
SILAC relies on the metabolic incorporation of non-radioactive heavy isotopes in the whole proteome of desired cell line, making all proteins from these cells easily distinguishable in the mass spectrometers from the proteins originating from control cells.
Multiply charged mass spectra of peptides and proteins in 2,5-dihydroxybenzoic acid matrix were readily obtained on two mass spectrometers from different manufacturers with sample introduction using melting point tubes.
We propose a structured ANOVA-type model that benefits both from the multiple spectral peaks produced by the mass spectrometer and from the collinear structure (Huopaniemi et al., 2009; Steuer, 2006) of metabolomic data.
The limits of detection (S/N = 3) ranged from 0.005 to 0.6 μmol l−1 for an ion-trap mass spectrometer and from 0.0004 to 0.08 μmol l−1 for a time-of-flight mass spectrometer.
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