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At 1∶60,000 PDGF to total protein ratio, PDGF peptides were not detected in the original solution after trypsin digestion for mass spectrometric analysis (PDGF concentration of 0.67 ng/mL), while eluates from the particles yielded a clear mass spectrometer detection spectra of the PDGF trypsin peptides.
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The bioremediation samples were analyzed on mass spectrometer for detection of the intermediates formed during bioremediation.
The samples of each concentration were analyzed on mass spectrometer for detection of intermediated formed during the bioremediation of anthracene.
The samples of each concentration were analyzed on a mass spectrometer for detection of intermediates formed during the bioremediation of anthracene.
After extraction, the lipids are often separated by various chromatographic methods such as thin layer chromatography (TLC), gas chromatography (GC), and high-performance liquid chromatography (HPLC), or lipids can be directly infused to a mass spectrometer for detection without separation.
The bioactive compounds of V. macrocarpon have been analyzed previously in fresh fruit and cranberry juice by high-performance liquid chromatography (HPLC), ultraviolet visible (UV/Vis) and mass spectrometer (MS) detection [ 4– 6].
In the dynamic mode, we show that mobility-selected ions can be switched into the alternative channel, and that various ion species can be independently selected based on their mobilities for time-of-flight mass spectrometer (TOF MS IMSS detection and mass analysis.
Next, the purified crosslinked samples were double digested with trypsin and Glu-C protease, fractionated by cation exchange chromatography and analysed by nano-scale reversed phase liquid chromatography coupled to a tandem mass spectrometer for detection and identification of crosslinked peptides.
Some of the ubiquitylated peptides that were not identified in their cognate unmodified form contained di-Gly-modified Lys residues at locations in the peptide that would result in tryptic peptides with m/z values below the mass spectrometer's detection range.
A new charge detection mass spectrometer that combines array detection and electrostatic ion trapping to repeatedly measure the masses of single ions is described.
M.T.: Since we are using antibody-based or mass spectrometer-based detection assays, it has been more or less invisible.
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