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Mass spec grade trypsin/Lys C mix and dithiothreitol (DTT) were purchased from Promega (Madison, WI).
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With colleague Nathalie Agar at Boston's Brigham and Women's Hospital, he already has demonstrated the feasibility of grading brain tumors using the lipid profiles they produce in a mass spectrometer (see "Mass Spec Imaging: From Bench to Bedside," scim.ag/1dCjmPx).ag/1dCjmPx
"We want to do the same thing with the mass spec".
How, though, to make a mass spec small enough and robust enough to operate underwater?
Besides the vacuum, miniaturizing a mass spec poses other difficulties, too.
For Verbeck, the primary challenge in making a smaller mass spec was electrical.
Precise points of modification can be determined by mass spec analysis after cleavage.
The GC-MS spectra were recorded on Varian Saturn (mass spec model).
Mass spec results are given as m/z in g/mol.
As more companies rely on mass specs to provide crucial data for quality control as well as proteomics and metabolomics investigations, "you're going to see more mass spec needs in industry," he asserts.
As with advanced mass spec imaging systems, this approach allows researchers to measure protein content cell by cell in situ.
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