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Analysis of Mps1 using phospho-specific antibodies or other means, combined with thorough functional analysis of single point mutants is needed to pinpoint functionally relevant phosphorylations on Mps1 and distinguish actual in vivo sites from mass spec artifacts.
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Precise points of modification can be determined by mass spec analysis after cleavage.
Besides the vacuum, miniaturizing a mass spec poses other difficulties, too.
For Verbeck, the primary challenge in making a smaller mass spec was electrical.
"We want to do the same thing with the mass spec".
How, though, to make a mass spec small enough and robust enough to operate underwater?
The GC-MS spectra were recorded on Varian Saturn (mass spec model).
Mass spec results are given as m/z in g/mol.
As more companies rely on mass specs to provide crucial data for quality control as well as proteomics and metabolomics investigations, "you're going to see more mass spec needs in industry," he asserts.
As with advanced mass spec imaging systems, this approach allows researchers to measure protein content cell by cell in situ.
"This is the next frontier of understanding proteomics data sets, and the mass spec is the key to that".
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