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Ion traps, for instance, are attractive candidates for miniaturization not only because they are so simple, but also because they have the built-in capacity for tandem mass spec analyses, enabling sophisticated structural analyses.
In multiple independent TAP purifications of Ccr4-Not subunits analyzed on SDS-PAGE or native gels followed by either one of two different mass spec analyses we identified the Mlp1, Mlp2, Nup2, and Nup60 components of the inner nuclear basket of the NPC [51], [52], [53].
Mass spec analyses show that Lysine-100 in fibrinogen Aα subunit reacts with the Cys-Gln thioester and forms an isopeptide bond with the glutamine side chain.
Similar(57)
Irrespective of the particular technology platform, be it Affymetrix Chip, miRNA profiles, mass spec proteomics, metabolomics or so on, the challenge of focusing analyses on the most informative markers is similar.
Edwards says, "AssayMAP is especially effective when used upfront of LC/MS analyses, delivering consistent samples that allow users working in biopharma and proteomics to achieve superior mass spec results".
Precise points of modification can be determined by mass spec analysis after cleavage.
"We want to do the same thing with the mass spec".
How, though, to make a mass spec small enough and robust enough to operate underwater?
Besides the vacuum, miniaturizing a mass spec poses other difficulties, too.
For Verbeck, the primary challenge in making a smaller mass spec was electrical.
The GC-MS spectra were recorded on Varian Saturn (mass spec model).
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