We have recently shown that glycans isolated from natural sources and labeled with fluorescent tags such as 2-AA and 2-AB using reductive amination are not only suitable for many separation techniques with fluorescence and mass-spectrometric detection (see " Separation methods"), but can also directly be used for covalent immobilization on a natural glycan microarray [ 202] (Fig. 9).
However, palmitoylation of the protein does not result in a large difference in relative molecular mass using gel electrophoretic separation techniques and cells carrying mutations in the CD95 palmitoylated site did not have an altered mobility in SDS-PAGE [28].
Advances in the design and efficiency of the interfaces that directly connect separation techniques with mass spectrometers have afforded new opportunities for monitoring, characterizing, and quantifying drug-related substances in APIs and pharmaceutical formulations.
Mass spectrometry, in combination with separation techniques, can play an important role in identifying and monitoring biomarkers in physiological fluids, which is a useful way of assessing drug efficacy and safety issues [ 32].
Post processing of dataset obtained using separation techniques hyphenated with mass spectrometry can allow automation of the recovery of target information, improve the visualization of the data or allow for mathematical separations of convoluted data.
Typically optical (e.g., Fourier transform infrared spectrometry), nuclear magnetic resonance (NMR) [19], [20], [21] and mass spectrometric techniques in combination with separation techniques, such as gas chromatography, high performance liquid chromatography (HPLC) and capillary electrophoresis, have been used for metabolomic studies [22], [23], [24], [25], [26].
Separation techniques coupled with mass spectrometry have allowed us to make many identifications (Nepi et al., 2009).
The purpose of this paper is to summarize recent progress accomplished by Health Canada's Food Allergen Research Program in enabling determination and confirmation of gluten protein markers in beverages such as beer products using a combination of protein separation techniques coupled with mass spectrometry.
Mass spectrometry (MS) in combination with modern separation techniques is one of the most powerful and versatile techniques for the structural analysis and characterization of carbohydrate interactions in microbial virulence.
Additionally, strategies for the analysis of tagged oligosaccharides using chromatographic and electromigrative separation techniques as well as mass spectrometry (MS) and their use in quantitation will be addressed.
