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The initial plume velocity and average ablated mass per pulse allow predicting the asymptotic NP size.
It has subsequently been shown that an infusion of kisspeptin-54 (0.01 nmol/kg/h (0.059 µg/kg/h) to 1 nmol/kg/h (5.9 µg/kg/h)) for 8 h in women with hypothalamic amenorrhoea can induce LH pulsatility with a 3-fold increase in LH pulse frequency and mass per pulse (Jayasena et al., 2013a).
Overall, the LH response to kisspeptin was more limited than that achieved in healthy men using the same protocol (George et al., 2011) with lower LH mass per pulse, although pulse frequency was normalized, consistent with complex neuropeptide interactions associated with KNDy neurone function rather than a linear hierarchy, as described above.
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The second major problem connected with the use of LA-ICP-MS for direct analysis of solid samples is the difference in the interaction between the laser beam and the sample surface observed for various matrices, causing changes in the mass of analyte ablated per pulse due to differences in the properties of the matrices investigated (e.g., absorptivity, reflectivity, and thermal conductivity).
It delivers < 3 ns duration pulses with an average per-pulse energy of 0.5mJ at 1.5 kHz pulse repetition rate.
Long helium pulses (∼100 s duration) and sufficient capability (1 2 kg of He per pulse) facilitate attainment of the steady-state regime in experiments, when 5 15 g/s mass flow rate of helium was scanned.
The probes deliver 3,000 volts of electrical current to the body, or 0.36 joules per pulse.
By contrast, a cardiac defibrillator operates with 360 joules per pulse on average, Mr. Tuttle said.
The tested parameters were the number of pulses, fluence per pulse and laser repetition rate.
The laser output per pulse was set to 0.1 to 2.0 mJ/pulse.
The pulse width of 10 ns and energy of 300 mJ per pulse have been used.
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