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In certain applications, large numbers of droplets may be deposited in arrays of multiple bursts, so the mass per burst may be the desired quantity.
Alternatively, determining the average mass per burst, as outlined in the pulsed burst method, accounts directly for mass differences in the droplets dispensed.
In these cases, eq 1 may still be applied, where the value of m is the mass per burst and N is the number of bursts dispensed.
Where small to moderate (10 to 1000) numbers of droplets are deposited together as a burst, the mass per burst may be the desired quantity.
The dispense system is programmed to eject a fixed number of droplets at each trigger signal, and trigger signals separated by 1-s delays are transmitted for at least 1 min. For this particular method, eq 2 is applied because this method is similar to the continuous droplet approach, except that now the value of m is the mass per burst.
Similar(54)
Transformations of bursting can be detected quantitatively by a sudden change of the number of spikes per burst.
In this region of bursting with a high number of spikes transforms into bursting with a drastically lower number of spikes per burst.
Cyan circles correspond to smaller number of spikes per burst (minimum of two spikes) and the largest dark red circle corresponds to the largest number of spike per burst (36 spikes in a burst).
Table 1 Signalling data per burst used in the DL-MAP.
The color of a circle represents the number of spikes per burst.
Preliminary results show that, the number of burst was increased by 154%, burst duration was increased by 35%, and the number of spikes per burst was increased by 124%, while interburst interval decreased by 44% with learning.
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