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Variable regions V1 and V2 of the 16S rRNA gene were then amplified using primers for bar-coded mass parallel sequencing using the FLX technology.
Here we present novel approach for contig anchoring based on mass parallel sequencing 3-dimmensional BAC pools prepared from MTP of physical map.
The progress in mass parallel sequencing methods (Mardis, 2008) allows generation of tags from a majority of genes via the RNA-seq approach (Wang et al., 2009) and identify genome-specific SNPs for detailed characterization of genome constitution.
However, such markers can be easily obtained by mass parallel sequencing and linearly ordered through the comparative analysis and synteny conservation with the sequenced model species [ 27].
A powerful approach for targeted development of markers became available thanks to the progress in mass parallel sequencing technology (Mardis 2008).
We demonstrate the utility of the novel approach for BAC contig anchoring based on mass parallel sequencing of three-dimensional pools prepared from minimum tilling path of physical map.
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With the current trend of mass parallel-sequencing technologies, SNPs will soon be discovered with ease, without requiring the use of predefined positions for their detection.
Current 454-pyrosequencing technology enables massive parallel sequencing.
TGE: Targeted genomic enrichment; MPS: Massively parallel sequencing.
The transcriptome was generated by paired-end sequencing of ~200bp cDNA fragments using Illumina parallel sequencing.
These irritating emails underscore an important point: massively parallel sequencing has arrived to the masses.
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