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Placing the inner cell mass in vitro under the finely tuned culture conditions prevents their imminent differentiation and allows cells to self-renew seemingly indefinitely whilst retaining their differentiation potential (Thomson et al., 1998).
However, at present, there is no established means of achieving mass in vitro germination and plantlet culture.
We believe that it will be possible to increase its population size by using mass in vitro seed germination to generate large numbers of plants to re-introduce into the wild (Wochok 1981).
Additionally, the 3-in-1 QCM exposure chamber, although designed to quantify deposited smoke mass in vitro, could potentially be used to assess other types of aerosol delivery to in vitro cultures, such as manufactured particles and fibres, some aerosolised cosmetics, household products, or pesticides.
27 One advantageous benefit of GLP-1 agonists, including dulaglutide, is a potential increase in β-cell mass in vitro via increased cellular regeneration and inhibition of apoptosis – as seen in young rodent models – which leads to slower progression of T2DM and a potential delay in the need for insulin therapy.
A method for mass in vitro seed germination and propagation will need to circumvent these requirements (Arditti and Ernst 1984; Sharma and Tandon 1987; Kumaria and Tandon 1991; Kondo et al. 1997; Gangaprasad et al. 1999; Nagaraju et al. 2003; Roy et al. 2011).
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However, during early expansion, ESCs obtained from the inner cell mass (ICM) in vitro continuously elongate their telomeres to a relatively stable level in a telomerase-dependent way, and ALT pathway could not be excluded in this process.
The aim of this study was to determine the correlation of inhaled drug mass between in vitro and ex vivo studies by testing aerosol deposition of various types of nebulizers.
Using mass spectrometry, in vitro phosphorylation assays and β2-syntrophin phosphomutants we found that phosphorylation of β2-syntrophin on S75 near the PDZ domain decreases its binding to ICA512 and correlates with increased granule motility, while phosphorylation of S90 has opposite effects.
Here we demonstrate that the c-Abl tyrosine kinase phosphorylates the widely expressed ΔNp63 α isoform and identify multiple sites by mass spectrometry in vitro and in vivo.
Although C. mastersii faces rapid destruction due to exploitation and also urbanization and habitat loss, there is, to date, no published information on how it may be effectively mass propagated in vitro.
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