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Scheme 2 Mass fragmentation pattern of the isatinic hydrazone.
The mass fragmentation pattern perfectly matched to the molecular weight of 4 − HMS.
The metabolites were identified based on accurate mass, fragmentation pattern, and retention time.
Furthermore, the mass fragmentation pattern (Scheme 2) supported the suggested structure of the ligand.
The identity of the reaction product was ascertained by mass fragmentation pattern and its comparison with the compound database NIST62- LIB available on the GC-MS instrument.
Myo-inositol hexa-trimethylsilyl ether was identified by comparing the mass fragmentation pattern with the database library NIST07 (MS Library Software, Thermo Scientific).
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Sterols were identified by reference to retention times and mass fragmentation patterns for known standards.
The products were identified by their characteristic mass fragmentation patterns (Lentz et al. 2001).
Moreover, MRM spectra of blanks with mass fragmentation patterns of CVA and IS were obtained to check the specificity of the method.
These heat of formation data were used as important evidence to define the unknown lipid structure as ceramide [BS] from the mass fragmentation patterns.
Total ion chromatograms (left) and the corresponding mass spectra (right) illustrate the product peaks at 17.10 and 18.68 min matching germacrene D-4-ol and δ-cadinol, respectively, in mass fragmentation patterns included in the database.
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