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Compounds were identified by comparing the retention times of resolved peaks with those of alkane and fatty acid standards, and by comparing the mass fragment pattern of each resolved peak to the National Institute of Standards and Technology (NIST) 2005 mass spectral library (NIST, Gaithersburg, MD) using the software GC/MS postrun analysis v2.3 (Shimadzu Scientific, Columbia, MD).
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On the other hand if a compound has been identified through an exact match to all 24 of its characteristic NMR peaks, or through matching the retention time, parent ion mass and mass fragment patterns of a known standard then obviously this compound's identity is on much more solid ground.
These results indicate that CCS can be used as an orthogonal analytical parameter alongside the traditional molecular identifiers of precursor accurate mass, fragment ion accurate mass, isotope pattern, and chromatographic retention time as a confirmation of metabolite identity to increase the confidence of identification.
Separated proteins are digested with enzymes such as trypsin, then the peptide mass fingerprinting is used to search sequence databases and to identify proteins that match the observed fragment pattern.
After H*Protein adsorption, the typical alkyl fragment pattern is complemented by peaks very characteristic for proteins, especially at even masses, which have been marked in Fig. 5b.
Lectionary for the Mass (fragment).
The PrPres banding pattern was essentially similar to that of vCJD (low molecular mass fragments and predominance of diglycoform species; Figure 1).
The genotypes were defined according to generated fragment patterns.
Fragment patterns were compared according to Tenover's criteria [ 35].
Two major strategies dominate cDNA fragment pattern-based transcriptomics.
The high mass range (m/ z 75 to 250) shows secondary ion fragments with the typical fragmentation pattern (successive cleavage of CH2) from the PDS precursor down to the intact pyridine tail group C5H5N+ at m/ z = 79.04.
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