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In the experiments with the AMS (experiments 17 22, Table S1) some compromises were made to have enough particle mass for detection with AMS: the initial droplet sizes were increased to 120 170 nm, sample flow rate in the flow tube was 0.4 L min 1, no sheath flow was used, and N was increased to 3600 43000 cm–3600 43000
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Serum samples were analyzed on Metabolon's integrated discovery platform consisting of gas and liquid chromatography for separation and mass spectrometry for detection and identification.
The bioremediation samples were analyzed on mass spectrometer for detection of the intermediates formed during bioremediation.
The samples of each concentration were analyzed on mass spectrometer for detection of intermediated formed during the bioremediation of anthracene.
The samples of each concentration were analyzed on a mass spectrometer for detection of intermediates formed during the bioremediation of anthracene.
After extraction, the lipids are often separated by various chromatographic methods such as thin layer chromatography (TLC), gas chromatography (GC), and high-performance liquid chromatography (HPLC), or lipids can be directly infused to a mass spectrometer for detection without separation.
We also used mass spectrometry for detection of IG20 in brain and spinal cord tissues of treated animals.
For example, hundreds of proteins can be identified in parallel with sensitive mass spectrometers for detection and analysis of individual peptides in complex protein mixtures.
This treatment allowed the identification of four additional adduction sites on Hsp90β, with a data-dependent LC-MS/MS method supplemented with an accurate inclusion mass list for detection on a Thermo LTQ-Orbitrap.
Next, the purified crosslinked samples were double digested with trypsin and Glu-C protease, fractionated by cation exchange chromatography and analysed by nano-scale reversed phase liquid chromatography coupled to a tandem mass spectrometer for detection and identification of crosslinked peptides.
We designed and prepared synthetic phospholipids that generate lyso-phosphatidylcholine products with a unique mass for convenient detection by LC MS in complex biological matrices.
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