The purified fraction was identified as a glutamate dehydrogenase (GDH) by peptide mass fingerprinting data.
Peptide mass fingerprinting analysis shows SCRII belongs to short-chain dehydrogenase/reductase family.
The identities of the eluted bands were confirmed by peptide mass fingerprinting as previously described.
Peptide mass fingerprinting [37] revealed that the purified protein was His6-tagged MutS2.
Proteins in the active fraction were identified by SDS-page and peptide mass fingerprinting.
The bands corresponding to purified rHLZ and commercial HLZ were removed from the gel for peptide mass fingerprinting (PMF).
MALDI TOF/TOF mass spectrometry was performed for peptide mass fingerprinting (PMF) as described [58] with some modifications.
After Coomassie staining, the band analogous to section 10 was cut and analyzed by peptide mass fingerprinting.
Proteins were identified by automated peptide mass fingerprinting using the Global Proteome Server Explorer software (Applied Biosystems).
Identification of the peptide was achieved by peptide mass fingerprinting.
The single protein band after purification was confirmed by the peptide mass fingerprinting (Yanxing, China).
