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Exact(6)
For instance, incorporation of the tryptophan analog A18 was tested using a template with 4 tryptophan codons (see supporting information Figure S1) and was unambiguously identified due to its +4 mass difference from the tryptophan containing peptide.
The contribution of the various components to the overall mass spectrum was then assessed by simulating spectra for all products identified based on mass difference from the intact particle.
Members of each MIDA template have a set mass difference from a "template initiator" ion, the lowest m/ z template member.
The mass difference from the HS holo form was 112.2 Da, consistent with the expected 112 Da shift from the piperazyl group.
The abundant matched b- and y- ions, accurate precursor ion mass, and expected mass difference from the SILAC pair observed in the spectrum correlated well with the low expectation value or p-value reported by algorithms.
Cell lysates were prepared, and for each protein a minimum of two SILAC-labeled tryptic peptides were unambiguously identified on the basis of their mass difference from endogenous peptides.
Similar(54)
The remaining three ions result from proton transfer and adduct formation reactions during methane PCI, with mass differences from the initiator of 58.07825, 86.10955, and 98.10955 Da.
Although it is a powerful tool, the applicability of mass spectrometry is limited, like chromatography, by the ability to resolve mass differences within the scaffold (small in the case of a protein) from mass differences from ligand-to-scaffold ratios (large in the case of a 3.5 kDa or larger polymeric ligand such as PEG).
ANOVA revealed significant differences among the treatments and the genotypes (Fig. 3A and 3B; ANOVA, F17,426 = 14.14, P < 0.001), but the larval mass differences differed from those of the pattern leaf area damaged.
This list was used to tag the corresponding peptide-related ion spectra on the basis of mass difference, deviation from the predicted elution time, and the match between the theoretical and observed isotopic envelopes.
But LC speciation remains also challenging due to the equal nature of the labelled and unlabelled compounds, which cannot be separated via molecular weight only, because of the low mass difference resulting from the attachment of the free label.
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Justyna Jupowicz-Kozak
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